Fig 2: Effect of Eimeria maxima infection (treatment) on MMP-2 (A), MMP-7 (B), MMP-9 (C), TIMP-2 (D), TIMP-3 (E), and ADAMTS8 (F) mRNA level in chicken jejunum, ileum and cecum at 7 (D7) or 10 (D10) day post-infection (PI). The expression level of control (C) birds of D7 was set to 100% and the other values are presented as % of C D7 data. Each value represents mean ± SEM of six birds. Different letters denote statistically significant (P < 0.05) differences between means within particular ileal tissue. Abbreviations: ADAMTS8, ADAM metallopeptidase with thrombospondin type 1 motif 8; IF, birds infected with Eimeria maxima oocytes; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase.

Fig 3: Effect of age (development) on ileal levels of mRNA for MMP-2 (A), MMP-7 (B), MMP-9 (C), TIMP-2 (D), TIMP-3 (E), and ADAMTS8 (F). The expression level of e19 (-48 h) birds was set to 100% and the other values are presented as % of the -48 h data. Each value represents mean ± SEM of six birds. Different letters denote statistically significant (P < 0.05) differences between means. Abbreviations: ADAMTS8, ADAM metallopeptidase with thrombospondin type 1 motif 8; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase.

Fig 4: Signaling Pathway Involved in the Aggressiveness of HCT116CCR6.(A, B) Western blotting analysis of Erk1/2 or phospho-Erk1/2 and Akt, phosphorylated Akt (Ser473) or phosphorylated Akt (Ser308) in HCT116CCR6 and HCT116Ctr cells. Values were expressed as fold changes relative to HCT116Ctr, and normalized to ß-actin. (C) Upregulated (FXYD5 and SYK) or down-regulated genes (CDH1, KISS1 and TIMP2) in HCT116CCR6 cells screening with a human tumor metastasis RT2 profiler PCR Array. (D) Western blotting analysis of changed FXYD5, SYK, CDH1, KISS1 and TIMP2 genes in HCT116CCR6 and HCT116Ctr cells. Values were expressed as fold changes relative to HCT116Ctr, and normalized to ß-actin.